Human ACL/PCL Cell Monolayer Culture
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Human Anterior or Posterior Cruciate Ligament (ACL or PCL) Cells, Monolayer CultureHealthy Donor, Osteoarthritis (OA+) or Rheumatoid Arthritis (RA+)


Tissue Procurement: Human ACL/PCL is collected from cadaveric donors within 48 hours of death or as surgical specimens from a relevant procedure. The tissue is harvested from the knee joint from both clinically “healthy, normal” donors and from those diagnosed with rheumatoid and osteoarthritis. Proper consent and serological information is obtained on all specimens.

 

Monolayer Culture: Ligament tissue is digested with an enzymatic treatment. The isolated cells are seeded onto a flask or multi-well plate in media supplemented with 10% FBS. The cells are maintained in a humidified incubator at 37oC and 5% COfor a minimum of 48 hours to promote cell adhesion and then shipped with cold packs for overnight delivery. The standard seeding density is 50,000 cells/cm2.

 

Quality Control: Tested negative for fungal and bacterial contamination.

 

 

  • Item #: CDD-H-2575

Human ACL, PCL Cell Monolayer Culture

Price: $650.00
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