Human IVD Monolayer Cultures

Product number

CDD-H-2700 Intervertebral disc NP culture

 

CDD-H-2701 Intervertebral disc AF culture

 

Features

· Flask or multi-well plate format: T-75 flask or 12, 24, 48 and 96-well

· Seeding density:100,000 cell/cm²

· Shipping media: 50:50 DMEM/Ham’s F12 supplemented with 10% FBS

· All cultures are guaranteed contamination-free

· Discounts apply for multiple plate orders

Tissue Procurement: Human intervertebral disc (IVD) tissue is collected from the spine of cadaveric donors within 24 hours of death. Proper consent and serological information is obtained on all specimens. The intervertebral disc tissue is harvested into two pools, the nucleus pulposus (NP) and annulus fibrosus (AF); intermediate tissues are discarded.

 

Monolayer Culture Processing: Disc tissues are treated with a sequential enzyme cocktail to isolate cells from surrounding matrices. The cells are counted and transferred to a flask or multi-well plate in 50/50 DMEM/F12 media supplemented with 10% FBS. The cells are maintained in a humidified incubator at 37^oC and 5% CO₂ for 48 hours to promote cell adhesion and then shipped with cold packs for overnight delivery.

Monolayer cultures: cells seeded onto a T-75 flask and a 96-well plate

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