Human Chondrocyte Monolayer Cultures

Product number

CDD-H-2600 Normal chondrocyte monolayer

 

CDD-H-2602 OA+ chondrocyte monolayer

 

CDD-H-2601 RA+ chondrocyte monolayer

 

Features

· Flask or multi-well plate format: T-75 flask or 12, 24, 48 and 96-well

· Seeding density:100,000 cell/cm²

· Shipping media: 50:50 DMEM/Ham’s F12 supplemented with 10% FBS

· All cultures are guaranteed contamination-free

· Discounts apply for multiple plate orders

Tissue Procurement: Human cartilage tissue is collected from cadaveric donors within 24 hours of death or as surgical specimens from a relevant procedure. The tissue is harvested from the surfaces of the knee joint from both clinically “normal” donors and from those diagnosed with rheumatoid and osteoarthritis. Proper consent and serological information is obtained on all specimens.

 

Monolayer Culture Processing: Harvested cartilage is treated with a sequential enzyme cocktail to isolate primary chondrocytes. The cells are counted and transferred to a flask or multi-well plate in 50/50 DMEM/F12 media supplemented with 10% FBS. The cells are maintained in a humidified incubator at 37^oC and 5% CO₂ for 48 hours to promote cell adhesion and then shipped with cold packs for overnight delivery.

Monolayer cultures: cells seeded onto a T-75 flask and a 96-well plate

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