Human Ligament Explant Culture, Healthy Donor

Human Anterior/Posterior Cruciate Ligament (ACL/PCL) Explant Culture, Healthy Donor

 

Tissue Procurement: Human ACL and PCL is collected from cadaveric donors with no history of joint disease or arthritis within 48 hours of death. Proper consent and serological information is obtained on all specimens.

 

Explant Processing: The ACL and PCL are aseptically dissected from surrounding tissues in the knee joint. The ligament is cut into small explants approximately 3mm in size. Around 48 explants can be generated from each the ACL and PCL for a total of 96 explants. Please note the sizes are estimated and it is recommended that wet weights be determined for normalization purposes. Explants are distributed into multi-well plates or in bulk format in media supplemented with 10% FBS. The cultures are shipped overnight with cold packs.

 

Wet Weight Determination: Each explant is blotted and weighed using an analytical balance. Wet weights are reported in milligrams in an Excel spreadsheet. Wet weights can be used to normalize data during culture analysis.

 

Histology Service: A ligament explant will be formalin-fixed, paraffin embedded and sectioned into slices 5 microns thick. Two unstained slides and one stained with H&E will be provided.  Additional shipping required.

 

Quality Control: Tested negative for fungal and bacterial contamination.

 

***This item requires fresh tissue. The order will be filled as soon as the appropriate donor tissue is available. Please inquire at 847.498.9634 to request an estimated wait time. 

 

  • Item #: CDD-H-2470

Human Ligament Explant Culture, Healthy Donor

Price: $650.00
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